ANALIZA MIKROBIOLOGICZNA I MOLEKULARNA DLA ZROZUMIENIA ZJAWISK W PORZUCONYM OTWORZE STUDZIENNYM

Abstract

MICROBIOLOGICAL AND MOLECULAR ANALYSIS FOR UNDERSTANDING PHENOMENA IN AN ABANDONED WATER WELLAbstract. Geomicrobiological studies were performed in the abandoned water well which still function in the national groundwater monitoring network. Extensive biofouling of cases and screens, as well as microbial mat, which completely fill-up the well at the depth of about 100 m, have been found. The microbiological analysis documented abundant microorganisms in water, while the molecular analysis of microbial mat revealed the coexistence of sulphate reducing and iron-sulphur cycle bacteria. The presence of such developed microbiological communities might indicate organic matter and SO42- and Fe2+ ions supply necessary for the growth of microorganisms found in the mat, which in turn would significantly affect the water chemistry.